isolation, antimicrobial susceptibility and meca gene analysis of methicillin-resistant staphylococcus aureus

نویسندگان

f. arefi

m. mohsenzadeh

j. razmyar

چکیده

the objective of this study was to determine the occurrence of antimicrobial resistance among staphylococcus aureus and to estimate the presence of methicillin-resistance in s. aureus (mrsa) isolates obtained by culture and polymerase chain reaction (pcr) methods. for this purposes, 100 iranian white and feta cheese samples collected from different suppliers were initially evaluated for the occurrence of s. aureus by culturing methods. the obtained isolates were subjected to disc diffusion antimicrobial susceptibility tests and a pcr method to detect the meca gene. out of the 100 cheese samples examined, 25 (25%) samples were contaminated with s. aureus with a mean of 5.74 ± 5.67 log cfu/g. out of the 25 isolates, 23 (92%) were found to be resistant to at least one antibiotic or more, tested by a disk diffusion method. the highest rate of antibiotic resistance was observed to penicillin g (92%) followed by ampicillin (73%) and cloxacillin (68%). none of the isolates was resistant to gentamycin and vancomycin. eight (34.78%) of the 23 s. aureus isolates were genotypically confirmed as mrsa. the results indicate that the presence of antimicrobialresistant strains of s. aureus in iranian cheese samples constitute a potential risk for human health. this calls for better control of the spread of antimicrobial resistant strains as well as cheese contamination sources.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Isolation, antimicrobial susceptibility and mecA gene analysis of methicillin-resistant Staphylococcus aureus

The objective of this study was to determine the occurrence of antimicrobial resistance among Staphylococcus aureus and to estimate the presence of methicillin-resistance in S. aureus (MRSA) isolates obtained by culture and polymerase chain reaction (PCR) methods. For this purposes, 100 Iranian white and feta cheese samples collected from different suppliers were initially evaluated for the occ...

متن کامل

ABSENCE OF mecA GENE IN METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS ISOLATES

Methicillin-resistant Staphylococcus aureus has emerged as a serious threat to public health, causing both hospital and community-associated infections. The gold standard for MRSA detection is the amplification of the mecA gene that codes for the production of the altered penicillin-binding protein (PBP2a) responsible for classical methicillin resistance. This work determined the nature of meth...

متن کامل

Epidemiology of mecA-Methicillin Resistant Staphylococcus aureus in Iran: A Systematic Review and Meta-analysis

Objective(s)Staphylococcus aureus is a prevalent pathogen worldwide. Methicillin resistant S. aureus (MRSA), which is usually multi-resistant in hospitals, has been a daunting challenge for clinicians for more than half a century. The aim of this systematic review and meta-analysis is to determine the relative frequency (RF) of MRSA in different regions of Iran.Materials and MethodsSearch terms...

متن کامل

Frequency and antimicrobial susceptibility pattern of methicillin resistant Staphylococcus aureus.

OBJECTIVE To determine the frequency of methicillin resistant Staphylococcus aureus and its antimicrobial susceptibility pattern in patients reporting to Combined Military Hospital (CMH), Pano Aqil. STUDY DESIGN A non-interventional, cross-sectional study. PLACE AND DURATION OF STUDY Pathology Laboratory, Combined Military Hospital (CMH), Pano Aqil, from December 2005 to January 2008. MET...

متن کامل

antibiotic susceptibility pattern among staphylococcus spp. with emphasis on detection of meca gene in methicillin resistant staphylococcus aureus isolates

background staphylococcus aureus is a major pathogen in hospital setting and in the community and causes a wide range of diseases. mrsa infection has recently become a serious problem in anti-microbial chemotherapy. the aim of the study was to detect and analyze the antibiotic diversity and isolation of methicillin resistance gene (meca) of s. aureus isolated from tehran hospitals as a rapid an...

متن کامل

In Vitro susceptibility of methicillin-resistant Staphylococcus aureus and methicillin-susceptible Staphylococcus aureus to a new antimicrobial, copper silicate.

The soluble copper silicate (CS) MIC of 100 strains of methicillin-resistant Staphylococcus aureus and 100 strains of methicillin-susceptible S. aureus (MSSA) was 175 mg Cu/liter. Bactericidal and postantibiotic effects (> or =1 h) were seen at 2x MIC and 4x MIC. The frequency of mutation was <10(-9), and serial passage could not extend growth beyond 1.6x MIC.

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian journal of veterinary research

ناشر: shiraz university

ISSN 1728-1997

دوره 15

شماره 2 2014

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023